{"product_id":"staphylococcus-aureus-methods-and-protocols-paperback","title":"Staphylococcus Aureus: Methods and Protocols - Paperback","description":"\u003cp\u003eby \u003cb\u003eKelly C. Rice\u003c\/b\u003e (Editor)\u003c\/p\u003e\u003cp\u003ePreface...Table of Contents...Contributing Authors...\u003cbr\u003e1. Measurement of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Pigmentation by Methanol ExtractionLeia E. Sullivan and Kelly C. Rice\u003cbr\u003e2. Analysis of Murein Hydrolases and Proteases through ZymographyNichole A. Seawell and Jeffrey L. Bose\u003cbr\u003e3. Detection and Quantification of Secreted Nuclease Activity in \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Culture SupernatantsRichard E. Wiemels, Rebecca A. Keogh, and Ronan K. Carroll\u003cbr\u003e4. Quantitative Hemolysis AssaysMiranda J. Ridder, Seth M. Daly, Pamela R. Hall, and Jeffrey L. Bose\u003cbr\u003e5. In Vitro Assay for Quantifying Clumping of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Heidi A. Crosby, Jakub M. Kwiecinski, and Alexander R. Horswill\u003cbr\u003e6. Measuring Staphylococcal Promoter Activities using a Codon-Optimized β-galactosidase ReporterChristina N. Krute, Nichole A. Seawell, and Jeffrey L. Bose\u003cbr\u003e7. Evaluation of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Antibiotic Tolerance using Kill Curve AssaysJessica N. Brandwein and Kelly C. Rice\u003cbr\u003e8. Fluorescence Polarization (FP) Assay for Measuring \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Membrane FluidityKiran B. Tiwari, Suranjana Sen, Craig Gatto, and Brian J. Wilkinson\u003cbr\u003e9. Quantification of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Biofilm Formation by Crystal Violet and Confocal Microscopy Adam B. Grossman, Dylan J. Burgin, and Kelly C. Rice\u003cbr\u003e10. Growth of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e using a Rotary Cell Culture System Matthew R. Hauserman and Kelly C. Rice \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e11. Time-Resolved Fluorescence Assay for Measuring Oxygen Consumption Rates in \u003ci\u003eStaphylococcus aureus\u003c\/i\u003eLogan L. Bulock, Jennifer L. Endres, and Marat R. Sadykov\u003cbr\u003e12. Quantifying \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Membrane Potential using Flow CytometryNeal D. Hammer\u003cbr\u003e13. Best Practices for Preparation of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e Metabolomics Samples Kimberly L. James and Kelly C. Rice\u003cbr\u003e14. A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm ProteinsMei G. Lei, Ravi Kr. Gupta, and Chia Y. Lee\u003cbr\u003e15. An Ex Vivo Model for Assessing Growth and Survivability of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e in Whole Human Blood Brittney D. Gimza, Stephanie M. Marroquin, and Lindsey N. Shaw\u003cbr\u003e16. Bone Marrow-Derived Macrophage Infection AssayMiranda J. Ridder, Mary A. Markiewicz, and Jeffrey L. Bose\u003cbr\u003e17. Measurement of Osteoblast Cytotoxicity Induced by S. aureus Secreted ToxinsCaleb Ford and James E. Cassat\u003cbr\u003e18. Human Skin In Vitro Colonization Model for a Skin Wound Infected by \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e BiofilmJenelle Chapman and Michael E. Olson\u003cbr\u003eSubject Index List...\u003cbr\u003e\u003ch3\u003eBack Jacket\u003c\/h3\u003e\u003cp\u003eThis volume details the phenotypic characterization of \u003ci\u003eStaphylococcus aureus\u003c\/i\u003e, with a focus on in vitro and ex vivo methodologies. The chapters in this book cover topics such as in vitro assessment of classical S. aureus virulence attributes; quantifying promoter activity using a S. aureus codon-optimized lacZ plasmid; biologically-relevant growth environments; metabolic and stress resistance assays; and in vivo and ex vivo models of host-pathogen interaction. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003eCutting-edge and authoritative, \u003ci\u003eStaphylococcus aureus: Methods and Protocols\u003c\/i\u003e is a valuable resource for anyone interested in this fascinating and developing field.\u003cbr\u003e\u003c\/p\u003e\u003cdiv\u003e\n\u003cstrong\u003eNumber of Pages:\u003c\/strong\u003e 162\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eDimensions:\u003c\/strong\u003e 0.37 x 10 x 7 IN\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003eIllustrated:\u003c\/strong\u003e Yes\u003c\/div\u003e\u003cdiv\u003e\n\u003cstrong\u003ePublication Date:\u003c\/strong\u003e July 17, 2022\u003c\/div\u003e","brand":"Books by splitShops","offers":[{"title":"Default Title","offer_id":42721578287167,"sku":"9781071615522","price":194.38,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0105\/8226\/1823\/files\/5a1df5486b19ac0b964c941958a5080e.webp?v=1765092852","url":"https:\/\/dhl-adrianne.myshopify.com\/products\/staphylococcus-aureus-methods-and-protocols-paperback","provider":"BBB","version":"1.0","type":"link"}